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protease inhibitor cocktail  (Thermo Fisher)


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    Thermo Fisher protease inhibitor cocktail
    Protease Inhibitor Cocktail, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease inhibitor cocktail/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    protease inhibitor cocktail - by Bioz Stars, 2026-05
    99/100 stars

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    Image Search Results


    Cellular uptake and lysosomal escape of CAMs. (a), (b) Fluorescence images and normalized fluorescence quantification of chondrocytes and meniscus cells after the uptake of different materials, as well as the cellular uptake efficiency results. red: fluorescently labeled materials; green: cell membrane; scale bar: 10 μm; n = 3; one-way ANOVA; ∗∗ represents p < 0.01; ∗∗∗ represents p < 0.0005; and ns represents no significant difference. (c, d) Flow cytometry analysis and normalized fluorescence quantification of chondrocytes (c) and meniscus cells (d) taking up CAMs after exposure to different inhibitors. n = 3; one-way ANOVA; ∗ represents p < 0.05; ∗∗ represents p < 0.01; ∗∗∗ represents p < 0.0005; ∗∗∗∗ represents p < 0.0001; and ns represents no significant difference. (e) Lysosomal escape of chondrocytes incubated with PF or PFMPCr/DS CAMs for 5 min, 1 h, or 6 h. Blue: nuclei; red: fluorescently labeled materials; green: lysosomes; scale bar: 10 μm. (f) Lysosomal escape of meniscus cells incubated with PF or PFMPCr/DS CAMs for 5 min, 1 h, or 6 h. Blue: nuclei; red: fluorescently labeled materials; green: lysosomes; scale bar: 10 μm.

    Journal: Bioactive Materials

    Article Title: Artificial mitochondria ameliorates osteoarthritis through restoring cellular energy metabolism homeostasis

    doi: 10.1016/j.bioactmat.2026.02.028

    Figure Lengend Snippet: Cellular uptake and lysosomal escape of CAMs. (a), (b) Fluorescence images and normalized fluorescence quantification of chondrocytes and meniscus cells after the uptake of different materials, as well as the cellular uptake efficiency results. red: fluorescently labeled materials; green: cell membrane; scale bar: 10 μm; n = 3; one-way ANOVA; ∗∗ represents p < 0.01; ∗∗∗ represents p < 0.0005; and ns represents no significant difference. (c, d) Flow cytometry analysis and normalized fluorescence quantification of chondrocytes (c) and meniscus cells (d) taking up CAMs after exposure to different inhibitors. n = 3; one-way ANOVA; ∗ represents p < 0.05; ∗∗ represents p < 0.01; ∗∗∗ represents p < 0.0005; ∗∗∗∗ represents p < 0.0001; and ns represents no significant difference. (e) Lysosomal escape of chondrocytes incubated with PF or PFMPCr/DS CAMs for 5 min, 1 h, or 6 h. Blue: nuclei; red: fluorescently labeled materials; green: lysosomes; scale bar: 10 μm. (f) Lysosomal escape of meniscus cells incubated with PF or PFMPCr/DS CAMs for 5 min, 1 h, or 6 h. Blue: nuclei; red: fluorescently labeled materials; green: lysosomes; scale bar: 10 μm.

    Article Snippet: Total protein was extracted using RIPA lysis buffer (C1053, Applygen, China) containing protease inhibitors (P1265-1, Applygen, China) and phosphatase inhibitors (P1260-1, Applygen, China).

    Techniques: Fluorescence, Labeling, Membrane, Flow Cytometry, Incubation